Fermentation of 3,4-C14- and 1-C14-Labeled Glucose by Clostridium Thermoaceticum

Fermentation of glucose-C14 and xylose-C14 by various strains of Leuconostoc mesenteroides.

Dissimilation of C14-labeled glucose by Serratia marcescens.

Glucose containing radioactive carbon has been utilized in the study of the metabolic distribution of the various carbon atoms during dissimilation by the following organisms: Leuconostoc mesenteroides, (Gunsalus and Gibbs, J. Biol. Chem. 194, 871, 1952); Pseudomonas saccharophila, (Entner and Doudoroff, J. Biol. Chem. 196, 853, 1952); Pseudomonas fluorescens (Lewis et al., J. Biol. Chem., 216,...

A New Type of Glucose Fermentation by Clostridium thermoaceticum.

The thermophilic fermentation of cellulosic material is generally regarded -as a result of the combined action of several species of bacteria, some of which attack the cellulose and others the sugars formed from the breakdown of the cellulose. Much effort has been spent on attempts to isolate the cellulose fermenters but very little attention has been paid to the associated sugar-fermenting org...

Fermentation of ribose-C14 by Lactobacillus pentosus.

In studies (l-3) on the synthesis in vivo of ribose by the tracer technique, it was desirable to have a rapid means of degrading the pentose for determination of the Cl4 distribution. The fermentation of pentose by Lactobacillus pentosus 124-2 seemed suitable for this purpose. Fred et al. (4) showed that this organism produces 1 mole each of lactic and acetic acids per mole of pentose utilized....

Synthesis of D-Glucose-1-C14 and D-Mannose-1-C14

Heretofore, radiochemical yields of sugars position-labeled with carbon-14 have been only 5 to 10 percent. This paper reports an improved cyanohydrin synthesis, and the preparation of D-glucose-1-C and D-mannose-1-C from D-arabinose in yields of about 50 percent. Low yields in prior syntheses appear to have been due to several inefficient steps, and to the fact that extension of the carbon chai...